There have been several discussions in this newsgroup about toluidine blue. They can be found at the following link: http://groups.google.com/group/bionet.plants.education/search?hl=en&group=bionet.plants.education&q=toluidine+blue&qt_g=Search+this+group A google search will provide many other sources, such as the following: BIOL2053: Hand sectioning and staining of sections http://www.cavehill.uwi.edu/FPAS/bcs/courses/Biology/BIOL2053/2053proj/biol2053sect.htm Creating Free Hand Sections of Plant Specimens http://dbs.umt.edu/courses/biol316/freehand.htm Lux A., Morita S., Abe J. and Ito K. 2005. An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond). 96(6):989-96. http://aob.oxfordjournals.org/cgi/content/full/96/6/989
